منابع مشابه
Assessing actin’s growth rate
At the beginning of the 1980s, researchers knew that actin fi laments were present in all cell types and were important for force generation and cell movement. But relatively little was known about how actin monomers assembled into fi laments. In fact, researchers didn’t even possess information as fundamental as the rate constants of monomer association and dissociation. In 1981, however, Tom ...
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The tissue and developmental specificities of the three Drosophila isoactins, originally identified in primary myogenic cultures and in the permanent Schneider L-2 cell line, have been investigated. Of these three isoactins (I, II, and III), actins I and II are stable and actin III is unstable. Two-dimensional polyacrylamide gel electrophoretic analyses of total cellular extracts after 1-h [(35...
متن کاملBacterial actins and their diversity.
For many years, bacteria were considered rather simple organisms, but the dogmatic notion that subcellular organization is a eukaryotic trait has been overthrown for more than a decade. The discovery of homologues of the eukaryotic cytoskeletal proteins actin, tubulin, and intermediate filaments in bacteria has been instrumental in changing this view. Over the past few years, we have gained an ...
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New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
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We have affinity-fractionated rabbit antiactin immunoglobulins (IgG) into classes that bind preferentially to either muscle or nonmuscle actins. The pools of muscle- and nonmuscle-specific actin antibodies were used in conjunction with fluorescence microscopy to characterize the actin in vascular pericytes, endothelial cells (EC), and smooth muscle cells (SMC) in vitro and in situ. Nonmuscle-sp...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2015
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.2085fta